EMPIAR-12834 - Cryo-electron microscopy of virus-like particle composed of PVA coat protein (in vitro assembly) [2232 multi-frame micrographs composed of 39 frames each in TIFF format]

EMPIAR-12834

Cryo-electron microscopy of virus-like particle composed of PVA coat protein (in vitro assembly) [2232 multi-frame micrographs composed of 39 frames each in TIFF format]

Publication:

Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles

Koritnik N, Kežar A, Kavčič L, Tušek Žnidarič M, Leonardi A, Pollari M, Mäkinen K, Podobnik M

(---)

DOI: 10.1038/s42003-025-09502-w

Related EMDB entry:

Deposited:

2025-05-22

Released:

2026-01-15

Last modified:

2026-01-15

Imageset size:

1.60 TB

Imageset DOI:

10.6019/EMPIAR-12834

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of PVA VLP (in vitro assembly)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2232

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.95, 0.95)

Details:

The movies were imaged (Glacios, 200 kV) in counting mode with Falcon3 (Thermo Fisher Scientific) direct electron detector at a nominal magnification of 150,000 with a total electron dose of 40 e-/Å2.

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Citations

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)

Ultrathin liquid cells for microsecond time-resolved cryo-EM.

Curtis WA, Wenz J, Krüger CR, Barrass SV, Drabbels M, Lorenz UJ. (2026)

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles.

Koritnik N, Kežar A, Kavčič L, Žnidarič MT, Leonardi A, De S, Pollari M, Mäkinen K, Podobnik M. (2026)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

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