EMPIAR-12816 - 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) [4216 multi-frame micrographs composed of 29 frames each in TIFF format]

EMPIAR-12816

70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) [4216 multi-frame micrographs composed of 29 frames each in TIFF format]

Publication:

Structural analysis of noncanonical translation initiation complexes

Mattingly JM, Nguyen HA, Roy B, Fredrick K, Dunham CM

Journal of Biological Chemistry 300 (2024) 107743-107743

PMID: 39222680

DOI: 10.1016/j.jbc.2024.107743

Related EMDB entry:

EMD-43930

Deposited:

2025-06-04

Released:

2025-07-30

Last modified:

2025-07-30

Imageset size:

2.95 TB

Imageset DOI:

10.6019/EMPIAR-12816

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of 70S initiation complex containing fMet-tRNA-fMet M1, initiation factor 2, and a CUG start codon

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4216

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.814, 0.814)

Details:

Micrographs are 29-frame movies with a pixel size of 0.814 angstroms and an accumulated dose of 50.0 electrons per square angstrom. Files in the included "xml" folder contain Thermo EPU-format image shift data which can be used for beam shift group splitting. Micrographs are provided as gain-corrected TIFFs.

Files:

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