EMPIAR-12816 - 70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) [4216 multi-frame micrographs composed of 29 frames each in TIFF format]

EMPIAR-12816

70S initiation complex (tRNA-fMet M1, initiation factor 2 + CUG start codon) [4216 multi-frame micrographs composed of 29 frames each in TIFF format]

Publication:

Structural analysis of noncanonical translation initiation complexes

Mattingly JM, Nguyen HA, Roy B, Fredrick K, Dunham CM

Journal of Biological Chemistry 300 (2024) 107743-107743

PMID: 39222680

DOI: 10.1016/j.jbc.2024.107743

Related EMDB entry:

EMD-43930

Deposited:

2025-06-04

Released:

2025-07-30

Last modified:

2025-07-30

Imageset size:

2.95 TB

Imageset DOI:

10.6019/EMPIAR-12816

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of 70S initiation complex containing fMet-tRNA-fMet M1, initiation factor 2, and a CUG start codon

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4216

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.814, 0.814)

Details:

Micrographs are 29-frame movies with a pixel size of 0.814 angstroms and an accumulated dose of 50.0 electrons per square angstrom. Files in the included "xml" folder contain Thermo EPU-format image shift data which can be used for beam shift group splitting. Micrographs are provided as gain-corrected TIFFs.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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