EMPIAR-12777 - Single particle cryo-EM aligned micrographs of bacterial 30S ribosome in complex with transcribing RNA polymerase [25906 micrographs in MRC format]

EMPIAR-12777

Single particle cryo-EM aligned micrographs of bacterial 30S ribosome in complex with transcribing RNA polymerase [25906 micrographs in MRC format]

Publication:

Molecular basis of mRNA delivery to the bacterial ribosome

Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-André C, Rappsilber J, Walter NG, Weixlbaumer A

Science 386 (---)

PMID: 38562847

DOI: 10.1126/science.ado8476

Related EMDB entry:

EMD-51579 EMD-51575 EMD-51574 EMD-51573 EMD-51578 EMD-51580 EMD-51576 EMD-51577 EMD-51572 EMD-51585

Deposited:

2025-03-21

Released:

2025-07-25

Last modified:

2025-07-25

Imageset size:

2.22 TB

Imageset DOI:

10.6019/EMPIAR-12777

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Aligned motion-corrected dose-weighted summed micrographs of 30S bacterial ribosome in complex with RNA polymerase

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

25906

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.84, 0.84)

Details:

Data represents aligned, motion corrected, dose-weighted micrographs. Each micrograph is the sum of 50 frames collected from Quantifoil grids on a 300 keV Titan Krios in counting mode using a Gatan K3 camera at 105K magnification and a pixel size of 0.84A. Total electron exposure on specimen level corresponds to 49.95 electrons per Å^2.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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