EMPIAR-12707 - Single particle cryo-EM micrographs and particles of OLE RNA dimer [multiple data sets in MRC format]

EMPIAR-12707

Single particle cryo-EM micrographs and particles of OLE RNA dimer [multiple data sets in MRC format]

Publication:

Naturally ornate RNA-only complexes revealed by cryo-EM

Kretsch RC, Wu Y, Shabalina SA, Lee H, Nye G, Koonin EV, Gao A, Chiu W, Das R

bioRxiv (2024)

DOI: 10.1101/2024.12.08.627333

Related EMDB entry:

EMD-48163

Deposited:

2025-04-15

Released:

2025-06-27

Last modified:

2025-06-27

Imageset size:

437.71 GB

Imageset DOI:

10.6019/EMPIAR-12707

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Aligned and dose-weight averaged micrographs of OLE RNA dimer.

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6324

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.954, 0.954)

Details:

The micrographs are the dose-weighted average by patch motion correction in cryosparc.

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Particle images of OLE RNA dimer.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

87716

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.954, 0.954)

Details:

Particle parameters are from the final C2 reconstruction of OLE. Local motion trajectories are provided in data/ole_particles/J767.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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