EMPIAR-12692 - Cryo-EM structure of E. coli MaeB acetyl-CoA bound form and the ME domain dimer [24546 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12692

Cryo-EM structure of E. coli MaeB acetyl-CoA bound form and the ME domain dimer [24546 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Divergent acetyl-CoA binding modes mediate allosteric inhibition of bacterial hybrid-type malic enzymes

Sassa M, Yamato H, Tanino H, Fukuda Y, Inoue T

The Journal of biological chemistry 301 (2025)

PMID: 41197718

DOI: 10.1016/j.jbc.2025.110887

Related EMDB entry:

EMD-62816 EMD-63599

Deposited:

2025-03-21

Released:

2026-03-06

Last modified:

2026-03-06

Imageset size:

4.38 TB

Imageset DOI:

10.6019/EMPIAR-12692

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Ec ACO, Ec ACO ME dimer

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

24546

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.87, 0.87)

Details:

- Microscope: CRYO ARM™ 300
- Acceleration voltage (kV): 300
- Pixel size (Å/pix): 0.87
- Nominal Defocus (nm): -700 to -2000
- Spherical aberration (Cs): 2.7
- Detector: Gatan K3 (6k × 4k)
- Average electron dose per image (e-/A2): 40
- Number of frames per image: 40
- Please use gain.mrc as a gain reference without rotation or flipping in cryoSPARC.

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