EMPIAR-12691 - Cryo-EM structure of E. coli MaeB holo form and the ME domain dimer [7099 multi-frame micrographs composed of 25 frames each in TIFF format]

EMPIAR-12691

Cryo-EM structure of E. coli MaeB holo form and the ME domain dimer [7099 multi-frame micrographs composed of 25 frames each in TIFF format]

Publication:

Divergent acetyl-CoA binding modes mediate allosteric inhibition of bacterial hybrid-type malic enzymes

Sassa M, Yamato H, Tanino H, Fukuda Y, Inoue T

The Journal of biological chemistry 301 (2025)

PMID: 41197718

DOI: 10.1016/j.jbc.2025.110887

Related EMDB entry:

EMD-63586 EMD-62540

Deposited:

2025-03-11

Released:

2026-03-06

Last modified:

2026-03-06

Imageset size:

1.46 TB

Imageset DOI:

10.6019/EMPIAR-12691

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Ec holo, Ec holo ME dimer

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7099

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: CRYO ARM™ 200
- Acceleration voltage (kV): 200
- Pixel size (Å/pix): 0.83
- Spherical aberration (Cs): 2.7
- Nominal Defocus (nm): -700 to -2200
- Detector: Gatan K3 (6k × 4k)
- Average electron dose per image (e-/A2): 40
- Number of frames per image: 40
- Please use gain.mrc as a gain reference without rotation or flipping in cryoSPARC.

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Citations

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