EMPIAR-12625
Cryogenic electron tomography reveals helical organization of lipoprotein lipase in storage vesicles [19 stitched maps in MRC format]
Publication:

Cryogenic electron tomography reveals helical organization of lipoprotein lipase in storage vesicles

Gunn KH, Wheless A, Calcraft T, Kreutzberger M, El-Houshy K, Egelman EH, Rosenthal PB, Neher SB

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Related EMDB entry:
Deposited:
2025-03-13
Released:
2025-12-07
Last modified:
2025-12-07
Imageset size:
10.84 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • stitched maps
1. Processed Tomograms of Vesicles containing Lipoprotein Lipase Filaments
Category:
stitched maps
Image format:
MRC
No. of images or tilt series:
19
Image size:
(1024, 1024)
Pixel type:
16 BIT FLOAT
Pixel spacing:
(7.86, 7.86)
Details:
These are the reconstructed tomograms containing gold labeled vesicles from a lipoprotein lipase (LPL) secretory vesicle data set. The tomograms were reconstructed in IMOD, binned 4 times, denoised using topaz, and corrected using IsoNet. The gold labeled vesicles were subsequently analyzed for the presence of LPL filaments.
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