EMPIAR-12546 - Human TRPM4 ion channel in MAASTY copolymer lipid nanodisc in a calcium-bound state [6308 multi-frame micrographs composed of 42 frames each in EER format]

EMPIAR-12546

Human TRPM4 ion channel in MAASTY copolymer lipid nanodisc in a calcium-bound state [6308 multi-frame micrographs composed of 42 frames each in EER format]

Publication:

MAASTY: a (dis)ordered copolymer for structural determination of human membrane proteins in native nanodiscs

Pugh CF, Feilen LP, Živković D, Præstegaard KF, Sideris C, Borthwick NJ, de Lichtenberg C, Bolla JR, Autzen HE

Nature communications 16 (2025)

PMID: 41372170

DOI: 10.1038/s41467-025-66208-7

Related EMDB entry:

EMD-52611

Deposited:

2025-02-03

Released:

2026-02-03

Last modified:

2026-02-03

Imageset size:

3.27 TB

Imageset DOI:

10.6019/EMPIAR-12546

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human TRPM4 ion channel in MAASTY copolymer lipid nanodisc in a calcium-bound state.

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

6308

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.725, 0.725)

Details:

Microscope: Titan Krios G2
Detector: Falcon4i
Acceleration voltage (kV): 300
Pixel size (Å/pix): 0.725
Spherical aberration (Cs): 2.7
Average electron dose per image (e-/A2): 52
Gain reference provided: 20230426_193941_EER_GainReference.gain

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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