EMPIAR-12534
Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import [multiple data sets in TIFF and MRC formats]
Publication:

Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import

Chang YT, Barad BA, Rahmani H, Zid BM, Grotjahn DA

bioRxiv (2024)

PMID: 39071314

Related EMDB entry:
Deposited:
2025-01-10
Released:
2025-03-17
Last modified:
2025-03-17
Imageset size:
764.19 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • tilt series
  • reconstructed volumes
1. Raw unaligned frame and metadata for each tilt in tilt-series acquired on CHX-treated S. cerevisiae cryo-FIB lamellae
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
462
Image size:
(5760, 4092)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.663, 1.663)
Details:
Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload/Frames folder. The number of frames per image varies from 38 to 42. Subfolder names with YTC041_1, YTC041_2, and YTC042_2 are datasets acquired on S. cerevisiae grown in YPG. Subfolder names with YTC043_1 and YTC043_2 are datasets acquired on S. cerevisiae grown in YPD.
Files:
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2. Unaligned tilt series stack of cryo-electron tomography acquired on CHX-treated S. cerevisiae cryo-FIB lamellae
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
38
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.663, 1.663)
Details:
The tilt series stacks (.st file) and rawtlt files (in Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload/PACE_mdoc folder. Tilt series stacks are then aligned in IMOD.
Files:
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3. Reconstructed tomograms of CHX-treated S. cerevisiae lamellae used for segmentation of mitochondria
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
38
Image size:
(682, 960)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(9.98, 9.98)
Details:
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload/Particle_lists folder.
Files:
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4. Raw unaligned frame and metadata for each tilt in tilt-series acquired on vehicle-treated S. cerevisiae cryo-FIB lamellae
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
492
Image size:
(5760, 4092)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.638, 2.638)
Details:
Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload_nonCHX/Frames folder. The number of frames per image varies from 39 to 41. Subfolder name with YTC009_3 is the dataset acquired on S. cerevisiae grown in YPG, and subfolder name with YTC025_2 is the dataset acquired on S. cerevisiae grown in YPD.
Files:
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5. Unaligned tilt series stack of cryo-electron tomography acquired on vehicle-treated S. cerevisiae cryo-FIB lamellae
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
49
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.638, 2.638)
Details:
The tilt series stacks (.st file) and rawtlt files (in EMPIAR_upload_nonCHX/Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload_nonCHX/mdocs folder. Tilt series stacks are then aligned in IMOD.
Files:
Loading...
6. Reconstructed tomograms of vehicle-treated S. cerevisiae lamellae used for segmentation of mitochondria
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
49
Image size:
(682, 960)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(15.83, 15.83)
Details:
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload_nonCHX/Particle_lists folder.
Files:
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