EMPIAR-12510 - Mouse LRRC8A(BRIL):D in GDN [multiple data sets in TIFF, MRCS and MRC formats]

EMPIAR-12510

Mouse LRRC8A(BRIL):D in GDN [multiple data sets in TIFF, MRCS and MRC formats]

Publication:

Assembly and lipid-gating of LRRC8A:D volume-regulated anion channels

Lurie A, Kern DM, Henn K, Brohawn SG

bioRxiv (2024)

DOI: 10.1101/2024.11.24.625074

Related EMDB entry:

EMD-47282 EMD-47283

Deposited:

2024-12-03

Released:

2025-01-21

Last modified:

2025-01-21

Imageset size:

3.01 TB

Imageset DOI:

10.6019/EMPIAR-12510

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - processed

Image Sets

1. Unaligned tif movies for LRRC8A(BRIL):D

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9730

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.048, 1.048)

Details:

Gain reference in .mrc form is included in the folder with the movies (data/Frames/). In RELION, the gain must be flipped upside down (1).

Files:

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2. Complete polished particles stack for LRRC8A(BRIL):D

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

8105

Image size:

(416, 416)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.048, 1.048)

Details:

Contains all polished particles (220,293) as 8,105 .mrcs files. The particle information is in the main data folder in RELION .star format (data/AllPolishedParticles.star). The particles can also be imported into cryoSPARC for processing. Note that this is the full polished particles stack, which was processed further in cryoSPARC to clean the stack and to separate the two conformations.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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3. Particles stack for LRRC8A(BRIL):D conformation 1

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

43907

Image size:

(416, 416)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.048, 1.048)

Details:

Final particles stack for LRRC8A(BRIL):D conformation 1, restacked from cryoSPARC.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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4. Particles stack for LRRC8A(BRIL):D conformation 2

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

46893

Image size:

(416, 416)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.048, 1.048)

Details:

Final particles stack for LRRC8A(BRIL):D conformation 2, restacked from cryoSPARC.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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