EMPIAR-12490 - Single particle reconstruction of LGTV with prME from TBEV [32123 multi-frame micrographs composed of 1314 frames each in EER format]

EMPIAR-12490

Single particle reconstruction of LGTV with prME from TBEV [32123 multi-frame micrographs composed of 1314 frames each in EER format]

Publication:

The influence of the pre-membrane and envelope proteins on structure, pathogenicity and tropism of tick-borne encephalitis virus

Rosendal E, Bisikalo K, Willekens SM, Lindgren M, Holoubek J, Svoboda P, Lappalainen A, Könighofer E, Mirgorodskaya E, Nordén R, Morini F, Rosenbaum W, Růžek D, Ahlgren U, Anastasina M, Merits A, Butcher SJ, Nilsson E, Överby AK

bioRxiv (2024)

DOI: 10.1101/2024.10.16.618642

Related EMDB entry:

EMD-50518

Deposited:

2024-06-19

Released:

2025-01-29

Last modified:

2025-01-29

Imageset size:

16.81 TB

Imageset DOI:

10.6019/EMPIAR-12490

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned EER movies of the purified chimeric LGTV(TBEV:prME)

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

32123

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.917, 0.917)

Details:

Micrographs collected of a Titan Krios 300 keV, equipped with a Falcon 4i. Spherical aberration 2.7, total dose 40. Gain reference in the main data folder. When building atomic model had scale issues, recalibrated voxel size of the map to 0.944 angstrom. CryoSPARC workflow available in the main data folder.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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