EMPIAR-12481 - Single particle cryo-EM dataset of soft-landed and rehydrated GroEL [multiple data sets in EER format]

EMPIAR-12481

Single particle cryo-EM dataset of soft-landed and rehydrated GroEL [multiple data sets in EER format]

Publication:

Cryo-EM Sample Preparation Using Soft-landing Electrospray Ion Beam Deposition and Laser Flash Melting

Barrass SV, Esser TK, Mowry NJ, Eriksson L, Hruby J, Seeley LT, Drabbels M, Baker LA, Rauschenbach S, Lorenz UJ

(---)

Related EMDB entry:

EMD-52284

Deposited:

2024-12-06

Released:

2025-12-22

Last modified:

2025-12-22

Imageset size:

20.69 TB

Imageset DOI:

10.6019/EMPIAR-12481

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of soft-landed and rehydrated GroEL - grid 2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

11817

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.736)

Details:

EER files from a Falcon 4i detector - raw micrographs, not motion corrected

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi-frame micrographs of soft-landed and rehydrated GroEL - grid 1

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

11240

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

EER files from a Falcon 4i detector - raw micrographs, not motion corrected

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned multi-frame micrographs of soft-landed and rehydrated GroEL - grid 2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

23057

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

EER files from a Falcon 4i detector - raw micrographs, not motion corrected

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

See all citations

Protein Data Bank Japan News

[2026-01-14]

503 new PDB entries have been released on 2026-01-14.

[2026-01-13]

[wwPDB] wwPDB Shared Responsibilities on Data Processing

[2026-01-08]

[wwPDB] Time-stamped Copies of PDB and EMDB Archives

[2025-12-17]

[wwPDB] 20,000 Structures Deposited to the PDB in 2025

[2025-12-04]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2025-11-19]

[wwPDB] Coming soon: wwPDB Data Processing Distribution Changes

[2025-10-30]

[wwPDB] Coming Soon: Remediation of Metalloprotein-containing PDB Entries and CCDs

[2025-09-26]

[wwPDB] Introducing the first 3DEM Model-Map percentile slider to the wwPDB validation report

[2025-07-17]

[wwPDB] Transitioning to PDBx/mmCIF and Extended PDB IDs

[2025-06-25]

[wwPDB] Extension to EMDB Accession Codes

See all news