EMPIAR-12447 - Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril [5652 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12447

Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril [5652 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril

Sharma K, Banerjee S, Savran D, Rajes C, Wiese S, Girdhar A, Schwierz N, Lee C, Shorter J, Schmidt M, Guo L, Fändrich M

Journal of molecular biology 435 (2023)

PMID: 37481159

DOI: 10.1016/j.jmb.2023.168211

Related EMDB entry:

EMD-14739

Deposited:

2024-11-13

Released:

2025-06-02

Last modified:

2025-06-02

Imageset size:

928.00 GB

Imageset DOI:

10.6019/EMPIAR-12447

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of full-length hnRNPA1 amyloid fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5652

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100

GIF-K2 counting
12s exposure 40 frames
total dose at the sample 44.90e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s
defocus range -1.2 to -2.5

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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