EMPIAR-12435 - Single Particle cryo-EM dataset of the conventional 50S ribosomal subunit used as control for shaped pulses [7416 multi-frame micrographs composed of 45 frames each in TIFF format]

EMPIAR-12435

Single Particle cryo-EM dataset of the conventional 50S ribosomal subunit used as control for shaped pulses [7416 multi-frame micrographs composed of 45 frames each in TIFF format]

Publication:

Laser flash melting cryo-EM samples to overcome preferred orientation

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ

Nature methods 22 (2025) 1880-1886

PMID: 40877464

DOI: 10.1038/s41592-025-02796-y

Related EMDB entry:

EMD-51750

Deposited:

2024-11-04

Released:

2025-07-23

Last modified:

2025-07-23

Imageset size:

953.51 GB

Imageset DOI:

10.6019/EMPIAR-12435

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of the conventional 50S ribosomal subunit used as control for shaped pulses

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7416

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.651, 0.651)

Details:

Data was collected in super-resolution mode and binned on the fly

Files:

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)