EMPIAR-12399 - Single Particle cryo-EM dataset of the deposited and revitrified 50S ribosomal subunit [9532 multi-frame micrographs composed of 693 frames each in EER format]

EMPIAR-12399

Single Particle cryo-EM dataset of the deposited and revitrified 50S ribosomal subunit [9532 multi-frame micrographs composed of 693 frames each in EER format]

Publication:

Laser flash melting cryo-EM samples to overcome preferred orientation

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ

Nature methods 22 (2025) 1880-1886

PMID: 40877464

DOI: 10.1038/s41592-025-02796-y

Related EMDB entry:

EMD-51749

Deposited:

2024-11-04

Released:

2025-07-25

Last modified:

2025-07-25

Imageset size:

2.48 TB

Imageset DOI:

10.6019/EMPIAR-12399

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of deposited and revitrified 50S ribosomal subunit

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

9532

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.658, 0.658)

Details:

---

Files:

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoWriter: a robotic solution for improved Cryo-EM grid preparation.

Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M. (2026)