EMPIAR-12327 - RNAP-TopoI complex on bubble scaffold [multiple data sets in TIFF and MRCS formats]

EMPIAR-12327

RNAP-TopoI complex on bubble scaffold [multiple data sets in TIFF and MRCS formats]

Publication:

DNA topoisomerase I acts as supercoiling sensor for transcription elongation in E. coli

Vidmar VV, Borde CB, Bruno LB, Takacs MT, Batisse CB, Saint-Andre CSA, Zhu CZ, Espeli OE, Lamour VL, Weixlbaumer AW

(---)

Related EMDB entry:

EMD-51261 EMD-51262 EMD-51260

Deposited:

2024-10-02

Released:

2025-11-18

Last modified:

2025-11-18

Imageset size:

1.69 TB

Imageset DOI:

10.6019/EMPIAR-12327

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - unprocessed

Image Sets

1. unaligned multi-frame movies of RNAP-TopoI on bubble scaffold

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6324

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.862, 0.862)

Details:

300 kV, Cs 0.01 mm, defocus range -0.8 – -2.5 um, total dose 54.03 e/A2
Gain reference file is provided in data/movies/gain.mrc

Files:

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2. picked particles of of RNAP-TopoI on bubble scaffold

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

6223

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.862, 0.862)

Details:

Particles were picked and extracted in WARP (Tegunov & Cramer, Nat. Methods 2019) as a part of on-the-fly processing pipeline at IGBMC, Strasbourg, France.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

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CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

Icecream: High-Fidelity Equivariant Cryo-Electron Tomography

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2025)

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