EMPIAR-12323
Unaligned multi-frame micrographs of the mu opioid receptor bound to a nanobody antagonist NbE [5939 multi-frame micrographs composed of 44 frames each in MRC format]
Publication:

Structural basis of μ-opioid receptor targeting by a nanobody antagonist

Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M

Nature communications 15 (2024)

PMID: 39384768

Related EMDB entry:
Deposited:
2024-09-04
Released:
2025-01-09
Last modified:
2025-01-09
Imageset size:
7.97 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of the µ-opioid receptor bound to a nanobody antagonist NbE
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
5939
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.9759, 0.9759)
Details:
1. All the images are raw data.

2. All the images are multi-frame micrographs composed of 44 frames each in MRC format.

3. All the images are acquired on a Thermo Scientific Talos Arctica Cryo-TEM at an accelerating voltage of 200 kV using a Falcon III direct electron detector at a nominal
magnification of 150,000x, resulting in a pixel size of 0.9759 Å.

4. Data were collected using EPU with a defocus range of −0.6 to −2.0 μm and a total electron dose of 40 e−/Å2
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