EMPIAR-12300 - Iota toxin Ib pore serine-clamp mutant [7080 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12300

Iota toxin Ib pore serine-clamp mutant [7080 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Serine clamp of Clostridium perfringens binary toxin BECb (CPILEb)-pore confers cytotoxicity and enterotoxicity

Yoshida T, Monma C, Ninomiya Y, Takiguchi S, Fujita S, Uchida Y, Sakoda N, Karginov VA, Kishikawa J, Yamada T, Tsuge H

(---)

Related EMDB entry:

EMD-64005 EMD-39544

Deposited:

2024-09-17

Released:

2025-07-31

Last modified:

2025-07-31

Imageset size:

1.37 TB

Imageset DOI:

10.6019/EMPIAR-12300

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for Iota toxin Ib pore serine-clamp mutant

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7080

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.675, 0.675)

Details:

Acceleration Voltage: 300 kV
Cs: 0.0938 mm (corrected by Cs corrector)
Dose per frame: 1 electron/angstrom^2
Frames: 50 frames
Energy Filter: 20 eV
Record Mode: K3 (not CDS mode)
Defocus Range: -0.6 μm to -1.8 μm.

gain.mrc as a gain reference without rotation and flipping

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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