EMPIAR-12240 - Cryo-EM structure of 1.51 Angstroms mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil) [multiple data sets in TIFF and MRC formats]

EMPIAR-12240

Cryo-EM structure of 1.51 Angstroms mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil) [multiple data sets in TIFF and MRC formats]

Publication:

Cryo-EM structure of mouse heavy-chain apoferritin resolved at 1.51 Å resolution in 290 nm gold foil holes (HexAuFoil)

Wang CH, Wu KP, Chang YC

(2024)

Related EMDB entry:

EMD-60915

Deposited:

2024-07-23

Released:

2024-09-03

Last modified:

2024-09-03

Imageset size:

3.40 TB

Imageset DOI:

10.6019/EMPIAR-12240

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame movies of mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9017

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.324, 0.324)

Details:

Unaligned multi-frame movies of mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil). The movies (60 frames) were collected using Titan Krios (Magnification 130,000x) and K3 Bio-Quantum in super-resolution model with energy filter set to 15 eV energy slit width. Pixel size is 0.324 Å/pixel in super-resolution model, accumulating around 52 electron per squared Angstrom in a 1.6 second exposure. The beam shifts (X and Y), as reported by EPU, for each micrograph are listed in the attached .xml file.
Gain reference is 240508_Gain_Ref.mrc.

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2. Aligned and dose-weighted micrographs of mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil) from the first 36 raw movie frames

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

9017

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.648, 0.648)

Details:

The aligned and dose-weighted micrographs of mouse heavy-chain apoferritin in 290 nm gold foil holes (HexAuFoil) are from the first 36 motion-corrected images derived from 60 raw movie frames (accumulating 52 electrons per square angstrom in a 1.6-second exposure). Pixel size is 0.648 Å/pixel, accumulating around 31 electron per squared Angstrom in 36 frames. The beam shifts (X and Y), as reported by EPU, for each micrograph are listed in the attached .xml file.

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