EMPIAR-12239 - Interleaved scanning electron microscopy: exploring native samples at the mesoscale. E.gracilis [multiple data sets in TIFF and MRC formats]

EMPIAR-12239

Interleaved scanning electron microscopy: exploring native samples at the mesoscale. E.gracilis [multiple data sets in TIFF and MRC formats]

Publication:

Reduction of SEM charging artefacts in native cryogenic biological samples.

Velazco A, Glen T, Klumpe S, Pennington A, Zhang J, Smith J, Bowles W, Glynn C, Kobylynska M, Fleck RA, Naismith JH, Kim JS, Darrow MC, Grange M, Kirkland AI

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Related EMDB entry:

Deposited:

2024-08-01

Released:

2024-11-21

Last modified:

2024-11-21

Imageset size:

249.72 GB

Imageset DOI:

10.6019/EMPIAR-12239

Experimental metadata:

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Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Tiff subframes from scan gen

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

21500

Image size:

(2048, 2048)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(6.34, 6.34)

Details:

---

Files:

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2. subframes to frame and cropped to remove flyback

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

215

Image size:

(1920, 1920)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(6.34, 6.34)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

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3. frames cropped and made of the subframe aligned with motiocor2

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

215

Image size:

(1920, 1920)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(6.34, 6.34)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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