EMPIAR-12228 - Cryo-EM images of B. megaterium gas vesicles in thin vitreous monolayers [4352 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-12228

Cryo-EM images of B. megaterium gas vesicles in thin vitreous monolayers [4352 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Cryo-EM structure of gas vesicles for buoyancy-controlled motility

Huber ST, Terwiel D, Evers WH, Maresca D, Jakobi AJ

Cell 186 (2023) 975-986.e13

PMID: 36868215

DOI: 10.1016/j.cell.2023.01.041

Related EMDB entry:

EMD-14238

Deposited:

2024-07-26

Released:

2025-02-03

Last modified:

2025-02-03

Imageset size:

2.35 TB

Imageset DOI:

10.6019/EMPIAR-12228

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of B.megaterium gas vesicles

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4352

Image size:

(11520, 8184)

Pixel type:

SIGNED BYTE

Pixel spacing:

(0.685, 0.685)

Details:

Unaligned multi-frame movies of B.megaterium gas vesicles (concentation: OD500=3.1) in superresolution mode from a K3 detector. Pixelsize is 1.37A, superres pixelsize is 0.685A. This pixel size was used for processing and in the publication. Subsequent work established that the pixelsize was not perfectly calibrated and should be closer to 1.341A.

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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