EMPIAR-12206 - Single-particle cryo-EM structure of the RNA chaperone Hfq in a translation repression complex Hfq-Crc-estA [13651 multi-frame micrographs composed of 47 frames each in TIFF format]

EMPIAR-12206

Single-particle cryo-EM structure of the RNA chaperone Hfq in a translation repression complex Hfq-Crc-estA [13651 multi-frame micrographs composed of 47 frames each in TIFF format]

Publication:

Translational regulation by Hfq-Crc assemblies emerges from polymorphic ribonucleoprotein folding

Dendooven T, Sonnleitner E, Bläsi U, Luisi BF

The EMBO journal 42 (2022)

PMID: 36504222

DOI: 10.15252/embj.2022111129

Related EMDB entry:

EMD-16265

Deposited:

2024-06-03

Released:

2025-03-20

Last modified:

2025-03-20

Imageset size:

4.29 TB

Imageset DOI:

10.6019/EMPIAR-12206

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Multiframe micrographs of the Hfq-Crc translation repression complex formed on estA(118)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13651

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Hfq/Crc translation repression complex formed on estA(118) present in micrographs. The images were taken using an FEI Titan Krios G2, 300kV, with an average electron dose per frame of 1.02 electrons per square Angstrom. Defocus -1.1 to -2.5um.

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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