EMPIAR-12162
SBF SEM images of a notoroid in longitudinal orientation containing TNE-ZsGreen+ notochord cells (Fig S26 part 1) [multiple data sets in DM4 and TIFF formats]
Publication:

Notochord and axial progenitor generation by timely BMP and NODAL inhibition during vertebrate trunk formation

Tiago Rito TR, Ashley R.G. Libby ARGL, Madeleine Demuth MD, Marie-Charlotte Domart MCD, Jake Cornwall-Scoones JCS, James Briscoe JB

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Deposited:
2024-03-01
Released:
2024-09-26
Last modified:
2024-09-26
Imageset size:
169.97 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Low magnification raw SBF SEM overview images (ROI00) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run02)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
88
Image size:
(7000, 12000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(590.0, 590.0)
Details:
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
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2. High magnification raw SBF SEM overview images (ROI01) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run02)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
88
Image size:
(12000, 12000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
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3. Low magnification raw SBF SEM overview images (ROI00) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run03)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
13
Image size:
(6000, 3000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(800.0, 800.0)
Details:
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 below. Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
4. High magnification raw SBF SEM overview images (ROI01) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run03)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
13
Image size:
(12000, 5932)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI01) linked to lower magnification images in ROI00 Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
5. Low magnification raw SBF SEM overview images (ROI00) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run04)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
168
Image size:
(6000, 3000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(800.0, 800.0)
Details:
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
6. High magnification raw SBF SEM overview images (ROI01) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run04)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
168
Image size:
(12000, 5932)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI01) linked to lower magnification images in ROI00 Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
7. Low magnification raw SBF SEM overview images (ROI02) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run04)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
168
Image size:
(8000, 5932)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI02) linked to lower magnification images in ROI00 Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
8. Low magnification raw SBF SEM overview images (ROI00) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run06)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
16
Image size:
(6000, 3000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(800.0, 800.0)
Details:
Low magnification overview SBF SEM images (ROI00), linked to higher magnification images in ROI01 and ROI02 below. Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
9. High magnification raw SBF SEM overview images (ROI01) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run06)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
16
Image size:
(8000, 6010)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI01) linked to lower magnification images in ROI00 Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
10. High magnification raw SBF SEM overview images (ROI02) of notoroid with TNE-Zs green notochord in longitudinal orientation (Run06)
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
16
Image size:
(8000, 6010)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(90.0, 90.0)
Details:
High magnification images (ROI02) linked to lower magnification images in ROI00 Imaged at 1.8kV, 2us dwell, with a 20um aperture and with FCC. 50nm slices.
Files:
Loading...
11. Survey images
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
4
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Survey images showing the relative position of the ROIs for each Run (Run 2, 3, 4 and 6).
Files:
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Files:
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