EMPIAR-12081 - Movies of PARP1-DNA Complexes [multiple data sets in TIFF and EER formats]

EMPIAR-12081

Movies of PARP1-DNA Complexes [multiple data sets in TIFF and EER formats]

Publication:

PARP1-HPF1 structure and dynamics on nicked DNA suggest a mechanism for acute and localized ADP-ribosylation

Sverzhinsky A, Xue H, Langelier MF, Muniz Corrêa MV, Del Mundo J, Classen S, Hammel M, Rothenberg E, Pascal JM

Nature communications 17 (2026)

PMID: 41698892

DOI: 10.1038/s41467-026-69375-3

Related EMDB entry:

EMD-48285 EMD-48313

Deposited:

2023-05-10

Released:

2026-03-30

Last modified:

2026-03-30

Imageset size:

27.18 TB

Imageset DOI:

10.6019/EMPIAR-12081

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of PARP1-DNA-EB47 on graphene by vitrobot

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

24130

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.069, 1.069)

Details:

The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.38 e-/Å^2/s with a total electron dose of 52.77 e-/Å^2.

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2. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by vitrobot

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

9643

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.751, 0.751)

Details:

The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.18 e-/Å^2/s with a total electron dose of 43.08 e-/Å^2.

Files:

This entry cannot be downloaded from PDBj.

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3. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8600

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.0691, 1.0691)

Details:

Files:

This entry cannot be downloaded from PDBj.

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4. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless (crosslinked) on graphene by vitrobot; tilted collection

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

15299

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.069, 1.069)

Details:

The movies were imaged (Titan Krios) at a nominal pixel resolution of 1.069 A/pix using a Gatan K3 direct electron detector (Thermo Fisher Scientific) in counting mode at a tilt of 30 degrees. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 26.2 e-/Å^2/s with a total electron dose of 83.82 e-/Å^2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

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5. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid1

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

9643

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.7511, 0.7511)

Details:

The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.47 e-/Å^2/s with a total electron dose of 44.81 e-/Å^2.

Files:

This entry cannot be downloaded from PDBj.

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6. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless on graphene by Chameleon; grid2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7445

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.7511, 0.7511)

Details:

The movies were imaged (Titan Krios) at a nominal pixel resolution of 0.7511 A/pix using a Falcon 4EC direct electron detector (Thermo Fisher Scientific) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 7.90 e-/Å^2/s with a total electron dose of 55.27 e-/Å^2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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7. Unaligned multi-frame micrographs of PARP1-DNA-EB47-HPF1-Timeless in open-hole by Chameleon

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4246

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.548, 0.548)

Details:

The movies were imaged (Talos Arctica) at a nominal pixel resolution of 0.548 A/pix using a K2 Summit direct electron detector (Gatan) in counting mode. Movies were collected using Leginon (Suloway et al., 2005) with a total electron dose of 50.44 e-/Å^2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

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