EMPIAR-12079
Single particle dataset of respiratory complex I from Paracoccus dentrificans in n-dodecyl-β-D-maltoside (DDM) [2278 multi-frame micrographs composed of 25 frames each in MRC format]
Publication:

Structure of the turnover-ready state of an ancestral respiratory complex I

Ivanov BS, Bridges HR, Jarman OD, Hirst J

Nature communications 15 (2024)

PMID: 39472559

Related EMDB entry:
Deposited:
2024-04-11
Released:
2025-01-16
Last modified:
2025-01-16
Imageset size:
353.26 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   3,167,619,367 kilobytes
Compressed size:   370,418,272 kilobytes

Note that a junk file (Thumbs.db) in the original deposition was removed.
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of respiratory complex I from Paracoccus dentrificans in n-dodecyl-β-D-maltoside (DDM)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2278
Image size:
(3710, 3838)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.07, 1.07)
Details:
Non-gain corrected micrographs were imaged using a Gatan K2 in counting mode with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 10 s exposures captured in 25 frames and a total dose of 48 electrons Å-2.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)