EMPIAR-12067 - Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA [6271 multi-frame micrographs composed of 38 frames each in TIFF format]

EMPIAR-12067

Single-particle cryo-EM structures of PNPase in complex with the RNA chaperone Hfq and regulatory RNA [6271 multi-frame micrographs composed of 38 frames each in TIFF format]

Publication:

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Dendooven T, Sinha D, Roeselová A, Cameron TA, De Lay NR, Luisi BF, Bandyra KJ

Molecular cell 81 (2021) 2901-2913.e5

PMID: 34157309

DOI: 10.1016/j.molcel.2021.05.032

Related EMDB entry:

EMD-12884

Deposited:

2024-03-20

Released:

2024-06-12

Last modified:

2024-06-12

Imageset size:

6.78 TB

Imageset DOI:

10.6019/EMPIAR-12067

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6271

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.065, 1.065)

Details:

PNPase-3'ETS(LeuZ)_Hfq present in micrographs. The images were taken using an FEI Titan Krios, 300kV, with an average electron dose per image of 53.6 electrons per square Angstrom. Defocus -1 to -2.5um.

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