EMPIAR-12048
Tomograms and the raw 2d tilt series of ER-nuclear envelope junctions of HeLa cells in early telophase [multiple data sets in TIFF and MRC formats]
Publication:

The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after open mitosis in mammalian cells

Bragulat-Teixidor H, Ishihara K, Szücs GM, Otsuka S

bioRxiv (2023)

Related EMDB entry:
Deposited:
2024-04-16
Released:
2024-07-04
Last modified:
2024-07-04
Imageset size:
334.10 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
  • reconstructed volumes
1. Tilt series of ER-nuclear envelope junctions of HeLa cells in early telophase
Category:
tilt series
Image format:
TIFF
No. of images or tilt series:
110
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(4.51, 4.51)
Details:
HeLa cells were instantly frozen using a high-pressure freezing machine (High Pressure Freezer Leica EM ICE equipped with the Coral Life workflow; Leica Microsystems) and freeze-substituted into Agar 100 Epoxy resin (Agar Scientific). As fiducial markers, 15 nm of gold-conjugated Protein A (Cytodiagnostics, AC-15-05-10) was absorbed on both sides of 250 nm sections. Sections were post-stained with 2% UA and lead citrate. Dual axis tilt series were acquired with a Tecnai G2 20 transmission electron microscope operated at 200 kV and equipped with an Eagle 4k HS CCD camera (FEI) by using the Serial EM software. Images were recorded over a -55° to 55° tilt range on average with an angular increment 1°.
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2. Reconstructed tomograms of ER-nuclear envelope junctions of HeLa cells in early telophase
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
110
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(4.51, 4.51)
Details:
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Files:
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