EMPIAR-12045 - CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET) [10860 multi-frame micrographs composed of 38 frames each in TIFF format]

EMPIAR-12045

CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET) [10860 multi-frame micrographs composed of 38 frames each in TIFF format]

Publication:

CryoET of β-amyloid and tau within postmortem Alzheimer's disease brain

Gilbert MAG, Fatima N, Jenkins J, O'Sullivan TJ, Schertel A, Halfon Y, Wilkinson M, Morrema THJ, Geibel M, Read RJ, Ranson NA, Radford SE, Hoozemans JJM, Frank RAW

Nature 631 (2024) 913-919

PMID: 38987603

DOI: 10.1038/s41586-024-07680-x

Related EMDB entry:

EMD-18990

Deposited:

2024-04-15

Released:

2024-09-03

Last modified:

2024-09-03

Imageset size:

1.40 TB

Imageset DOI:

10.6019/EMPIAR-12045

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame Tif movies of extracted amyloid fibrils from post-mortem human AD patient brain

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10860

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Movie stacks are gain corrected but unaligned, 10,860 movies, each containing 38 tiff frames with a dose per frame of ~1.15 e-/Å2. The total cumulative dose for each movie is ~44 e-/Å2 over a 4 s exposure. Predominantly Tau fibrils present, also meshes of abeta fibrils and a small percentage of fibrils that resemble TMEM106B from 2D class averages.

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This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

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Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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