EMPIAR-12045 - CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET) [10860 multi-frame micrographs composed of 38 frames each in TIFF format]

EMPIAR-12045

CryoEM movies of human tau amyloid fibrils extracted from an AD patient brain (in association with in situ cryoET) [10860 multi-frame micrographs composed of 38 frames each in TIFF format]

Publication:

CryoET of β-amyloid and tau within postmortem Alzheimer's disease brain

Gilbert MAG, Fatima N, Jenkins J, O'Sullivan TJ, Schertel A, Halfon Y, Wilkinson M, Morrema THJ, Geibel M, Read RJ, Ranson NA, Radford SE, Hoozemans JJM, Frank RAW

Nature 631 (2024) 913-919

PMID: 38987603

DOI: 10.1038/s41586-024-07680-x

Related EMDB entry:

EMD-18990

Deposited:

2024-04-15

Released:

2024-09-03

Last modified:

2024-09-03

Imageset size:

1.54 TB

Imageset DOI:

10.6019/EMPIAR-12045

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame Tif movies of extracted amyloid fibrils from post-mortem human AD patient brain

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10860

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Movie stacks are gain corrected but unaligned, 10,860 movies, each containing 38 tiff frames with a dose per frame of ~1.15 e-/Å2. The total cumulative dose for each movie is ~44 e-/Å2 over a 4 s exposure. Predominantly Tau fibrils present, also meshes of abeta fibrils and a small percentage of fibrils that resemble TMEM106B from 2D class averages.

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Citations

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