EMPIAR-12036 - Structure of an ALYREF-exon junction complex hexamer [multiple data sets in EER and MRC formats]

EMPIAR-12036

Structure of an ALYREF-exon junction complex hexamer [multiple data sets in EER and MRC formats]

Publication:

mRNA recognition and packaging by the human transcription-export complex

Pacheco-Fiallos B, Vorländer MK, Riabov-Bassat D, Fin L, O'Reilly FJ, Ayala FI, Schellhaas U, Rappsilber J, Plaschka C

Nature 616 (2023) 828-835

PMID: 37020021

DOI: 10.1038/s41586-023-05904-0

Related EMDB entry:

EMD-14803

Deposited:

2024-01-16

Released:

2024-05-20

Last modified:

2024-05-20

Imageset size:

6.59 TB

Imageset DOI:

10.6019/EMPIAR-12036

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed
reconstructed volumes

Image Sets

1. Unaligned multi-frame micrographs of the ALYREF-EJX hexamer in EER format

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7941

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.945, 0.945)

Details:

Raw EER files from Falcon 4 detector, acquired with EPU and a total dose of 40 e-/Å2

Files:

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2. Motion-corrected and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

7941

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.945, 0.945)

Details:

Motion-corrected and dose-weighted micrographs

Files:

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3. Motion-corrected micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

7941

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.945, 0.945)

Details:

Motion-corrected micrographs without dose-weighting, used for CTF estimation

Files:

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4. Final particles used for reconstruction

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

1564602

Image size:

(320, 320)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(0.945, 0.945)

Details:

Final particles used for reconstrution

Files:

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5. CryoSPARC job of the final 3D reconstruction

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

1564602

Image size:

(None, None)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(None, None)

Details:

CryoSPARC job of the final 3D reconstruction

Files:

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6. Gainref file for motion correction of compressed movies

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.945, 0.945)

Details:

Gainref file for motion correction

Files:

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Browse All Files

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