EMPIAR-12032
2.08A Apoferritin Structure Solved Using an Indirect Scintillator-Coupled CMOS Detector at 300 kV [1893 multi-frame micrographs composed of 23 frames each in MRCS format]
Publication:

High-Resolution Single Particle Analysis Using an Indirect Scintillator-Coupled CMOS Detector at 300 kV

Aramaki S, Yoshida Y, Tanihara T, Oyama K, Otsuki K, Terada Y, Matsunaga N, Ohdo S, Mayanagi K

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Related EMDB entry:
Deposited:
2024-04-03
Released:
2024-06-13
Last modified:
2024-06-13
Imageset size:
6.28 TB
Imageset DOI:
Experimental metadata:
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Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Imageset for Apoferritin
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
1893
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.608, 0.608)
Details:
- JEOL CRYOARM 300 II
- 300 kV, cold FEG
- 20 eV slit
- 4.04 e/A2/frame
- The dataset was collected with 5x5 image shift. The file ends with "... [000-024].mrc.bz2" corresponds to the spot scan location.
- A subset of micrographs (data/EMD-39481_filelist.txt) was used to produce EMD-39481. Other micrographs are equally valid but have not been processed due to computational issues.
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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)
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