EMPIAR-12010 - Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation [multiple data sets in MRC and MRCS formats]

EMPIAR-12010

Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation [multiple data sets in MRC and MRCS formats]

Publication:

Initiation factor 3 bound to the 30S ribosomal subunit in an initial step of translation

Uday AB, Mishra RK, Hussain T

Proteins (2023)

PMID: 38148682

DOI: 10.1002/prot.26655

Related EMDB entry:

EMD-36619 EMD-36620

Deposited:

2024-03-24

Released:

2024-05-03

Last modified:

2024-05-03

Imageset size:

751.05 GB

Imageset DOI:

10.6019/EMPIAR-12010

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Raw_movies/*/*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) in each folder during processing.
Note that movies in the Movie2 folder of the original deposition had been corrected by three gain references,
so we divided them into three folders.

Original size:   5,077,103,148 kilobytes
Compressed size:   758,400,164 kilobytes

Contains:

micrographs - multiframe
picked particles - multiframe - processed

Image Sets

1. Unaligned multi-frame movies of the 30S-IF3 complex

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4564

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.2, 1.2)

Details:

The set consists of unaligned multi-frame movies. The two sub-directories corresponds to two sets of movies, each processed seperately and then combined at the end for the final reconstruction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Polished particles of 30S-IF3 complex

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

145664

Image size:

(320, 320)

Pixel type:

16 BIT FLOAT

Pixel spacing:

(1.2, 1.2)

Details:

Particles used for the final reconstruction of 30S-IF3 map. The two subdirectories correspond to two sets of particles picked from the corresponding two sets of movies. Each set was processed seperately and combined at the end for final reconstruction.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

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Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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