EMPIAR-11958 - Volume correlative light X-ray and electron microscopy (CLXEM) of mouse cremaster muscle endothelial cells [multiple data sets in TIFF format]

EMPIAR-11958

Volume correlative light X-ray and electron microscopy (CLXEM) of mouse cremaster muscle endothelial cells [multiple data sets in TIFF format]

Publication:

Volume correlative light X-ray and electron microscopy (CLXEM) of endothelial cells in mouse tissue

Reglero-Real N, Pérez-Gutiérrez L, Saleeb RS, Nourshargh S, Collinson L, Yoshimura A

STAR Protocols (---)

DOI: 10.1016/j.xpro.2024.103257

Related EMDB entry:

Deposited:

2024-02-18

Released:

2024-09-03

Last modified:

2024-09-03

Imageset size:

79.21 GB

Imageset DOI:

10.6019/EMPIAR-11958

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
micrographs - multiframe

Image Sets

1. X-ray micro-computed tomography (µCT) of resin-embedded mouse cremaster muscle tissue

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

Image size:

(1004, 1024)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(74181.0, 74181.0)

Details:

Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.

Files:

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2. X-ray µCT of resin-embedded mouse cremaster muscle tissue after first trimming around the region of interest

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

Image size:

(992, 1015)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(32635.0, 32635.0)

Details:

Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. X-ray µCT of resin-embedded mouse cremaster muscle tissue after final trimming before SBF-SEM

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

Image size:

(992, 1015)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(10313.0, 10313.0)

Details:

Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. SBF-SEM of the target endothelial cells identified by correlation of X-ray and light microscopy

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(9348, 13946)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(84.4, 500.0)

Details:

Data set of two SBF SEM acquisitions combined, aligned and processed as a tiff stack at the original resolution of acquisition.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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