EMPIAR-11958
Volume correlative light X-ray and electron microscopy (CLXEM) of mouse cremaster muscle endothelial cells [multiple data sets in TIFF format]
Publication:

Volume correlative light X-ray and electron microscopy (CLXEM) of endothelial cells in mouse tissue

Reglero-Real N, Pérez-Gutiérrez L, Saleeb RS, Nourshargh S, Collinson L, Yoshimura A

STAR Protocols (---)

Related EMDB entry:
Deposited:
2024-02-18
Released:
2024-09-03
Last modified:
2024-09-03
Imageset size:
79.21 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
  • micrographs - multiframe
1. X-ray micro-computed tomography (µCT) of resin-embedded mouse cremaster muscle tissue
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1004, 1024)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(74181.0, 74181.0)
Details:
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
Files:
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2. X-ray µCT of resin-embedded mouse cremaster muscle tissue after first trimming around the region of interest
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(32635.0, 32635.0)
Details:
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
Files:
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3. X-ray µCT of resin-embedded mouse cremaster muscle tissue after final trimming before SBF-SEM
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(992, 1015)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(10313.0, 10313.0)
Details:
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
Files:
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4. SBF-SEM of the target endothelial cells identified by correlation of X-ray and light microscopy
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(9348, 13946)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(84.4, 500.0)
Details:
Data set of two SBF SEM acquisitions combined, aligned and processed as a tiff stack at the original resolution of acquisition.
Files:
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