EMPIAR-11945 - Cryo-ET tilt series of untreated FIB-milled E. coli K-12 cells [multiple data sets in MRC format]

EMPIAR-11945

Cryo-ET tilt series of untreated FIB-milled E. coli K-12 cells [multiple data sets in MRC format]

Publication:

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Khusainov I, Romanov N, Goemans C, Turoňová B, Zimmerli CE, Welsch S, Langer JD, Typas A, Beck M

Nature communications 15 (2024)

PMID: 38839776

DOI: 10.1038/s41467-024-49084-5

Related EMDB entry:

EMD-18037 EMD-18036

Deposited:

2024-02-29

Released:

2024-06-19

Last modified:

2024-06-19

Imageset size:

77.27 GB

Imageset DOI:

10.6019/EMPIAR-11945

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo-ET tilt series of untreated FIB-milled E. coli K-12 cells dataset 1

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.697, 1.697)

Details:

The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -62 to +46 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.

Files:

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2. Cryo-ET tilt series of untreated FIB-milled E. coli K-12 cells dataset 2

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.697, 1.697)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Cryo-ET tilt series of untreated FIB-milled E. coli K-12 cells dataset 3

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.697, 1.697)

Details:

The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -9 degree pre-tilt and covering a range from -63 to +45 degrees, with a tilt increment of 3 degrees. Each wedge includes 2 tilts.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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