EMPIAR-11875
Human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif [multiple data sets in TIFF and DM4 formats]
Publication:

Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex

Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M

Nature structural & molecular biology 25 (2018) 135-138

PMID: 29358758

Related EMDB entry:
Deposited:
2024-01-24
Released:
2024-02-06
Last modified:
2024-02-06
Imageset size:
1.25 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(7676, 7420)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.058, 1.058)
Details:
FEI Titan Krios microscope operated at 300 kV and equipped with a Gatan K2 Summit detector and a Gatan Quantum GIF LS energy filter. Micrographs were collected in super-resolution mode and fourier-cropped on-the-fly with FOCUS software, consisted of 50 frames for a total exposure time of 10 s and a total dose of 80 e-/Å2. The defocus ranged between –2.2 and –1 μm. Data collected with Serial EM.
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2. Gain reference
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
1
Image size:
(7676, 7420)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.058, 1.058)
Details:
Gain reference
Files:
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Files:
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)