EMPIAR-11849 - FIB-SEM dataset showing localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7) [564 reconstructed volumes in TIFF format]

EMPIAR-11849

FIB-SEM dataset showing localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7) [564 reconstructed volumes in TIFF format]

Publication:

Localization of a Golgi matrix protein GM130 in human hepatocellular carcinoma cell (Huh-7)

Belevich I, Jokitalo E

(---)

Related EMDB entry:

Deposited:

2024-01-08

Released:

2024-02-15

Last modified:

2024-02-15

Imageset size:

1.14 GB

Imageset DOI:

10.6019/EMPIAR-11849

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. FIB-SEM dataset showing localization of a Golgi matrix protein GM130 (purple) in human hepatocellular carcinoma cell (Huh-7)

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

564

Image size:

(3269, 1397)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(40.0, 40.0)

Details:

Huh7, a human hepatoma-derived cell was immunostained using antibody against Golgi matrix protein GM130 and imaged using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) with voxels of 4.0 x 4.0 x 8.0 nm.

Immunolabeling as described in
Lak B, Li S, Belevich I, Sree S, Butkovic R, Ikonen E, Jokitalo E. Specific subdomain localization of ER resident proteins and membrane contact sites resolved by electron microscopy. Eur J Cell Biol. 2021 Sep-Nov;100(7-8):151180. doi: 10.1016/j.ejcb.2021.151180. Epub 2021 Oct 4

Stained using ferrocyanide-reduced osmium + thiocarbohydrazide + osmium (OTO) method

Purified mouse anti-GM130 was from BD biosciences.

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