These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector with a Selectris energy filter with a 10eV slit. Over the collection, the sample was exposed evenly to 60 electrons per angstrom squared with a total exposure time of 3.61 seconds. Desired defocuses ranged from -1.2 to -2.0 nm. The exposures are gain corrected.
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Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/HCoV-NL63_SL5//particles/conformation*.cs for the two confromations.
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