EMPIAR-11796
Single-Particle Cryo-EM of Apoferritin at Various Tilts [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection

Aiyer S, Baldwin PR, Tan SM, Shan Z, Oh J, Mehrani A, Bowman ME, Louie G, Passos DO, Đorđević-Marquardt S, Mietzsch M, Hull JA, Hoshika S, Barad BA, Grotjahn DA, McKenna R, Agbandje-McKenna M, Benner SA, Noel JAP, Wang D, Tan YZ, Lyumkis D

bioRxiv (---)

Deposited:
2023-11-27
Released:
2024-07-04
Last modified:
2024-07-04
Imageset size:
302.12 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - multiframe - processed
1. Unaligned multiframe movies of apoferritin collected at various tilts
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
637
Image size:
(4092, 5760)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.83, 0.83)
Details:
Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 195 frames were used, pixel size is 0.830 Å/pixel, total dose of 44 e-/Å2. Gain reference is 22oct13a_13123849_01_4092x5760_norm_0.mrc.
Files:
Loading...
2. Aligned and dose-weighted micrographs of apoferritin collected at various tilts
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
637
Image size:
(4092, 5760)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.83, 0.83)
Details:
Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.
Files:
Loading...
3. Polished and equalized particles of apoferritin collected at various tilts
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
7
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.83, 0.83)
Details:
Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.
Files:
Loading...
Files:
Loading...

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)