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EMPIAR-11795
CryoEM movies of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx [multiple data sets in MRC format]
Publication:

Structures of the nitrogenase complex prepared under catalytic turnover conditions

Rutledge HL, Cook BD, Nguyen HPM, Herzik Jr MA, Tezcan FA

Science (New York, N.Y.) 377 (2022) 865-869

PMID: 35901182

Related EMDB entry:
EMD-26764
Deposited:
2023-08-12
Released:
2024-01-15
Last modified:
2024-01-15
Imageset size:
1.74 TB
Imageset DOI:
10.6019/EMPIAR-11795
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (set*/movies/FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   5,722,022,736 kilobytes
Compressed size: 1,411,497,229 kilobytes
Contains:
  • micrographs - multiframe
  • micrographs - single frame
Image Sets
1. Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2198
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.815, 0.815)
Details:
Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames).
Files:
Loading...
2. Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx.
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2198
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.815, 0.815)
Details:
Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames).
Files:
Loading...
3. Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1718
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.815, 0.815)
Details:
Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames).
Files:
Loading...
4. Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1718
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.815, 0.815)
Details:
Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames).
Files:
Loading...
5. Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx at 15 degree tilt
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2211
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.815, 0.815)
Details:
Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 15 degrees.
Files:
Loading...
6. Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx at 15 degree tilt
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2211
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.815, 0.815)
Details:
Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 15 degrees.
Files:
Loading...
7. Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx at 25 degree tilt
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2103
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.815, 0.815)
Details:
Unaligned multi-frame micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 25 degrees.
Files:
Loading...
8. Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx at 25 degree tilt
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2103
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.815, 0.815)
Details:
Aligned micrographs of nitrogenase (MoFeP + FeP) from Azotobacter vinelandii under catalytic turnover conditions with inhibitor BeFx collected on a Titan Krios G3 (Thermo Fisher Scientific) operating at 300 keV equipped with a Gatan BioContinuum energy filter. Images were collected at a magnification of 165,000x in EF-TEM mode (0.815 Å calibrated pixel size) on a Gatan K2 detector using a 20-eV slit width and a cumulative electron exposure of ~65 electrons/Å2 (50 frames). Stage was tilted at 25 degrees.
Files:
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