EMPIAR-11784 - SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state [multiple data sets in EER format]

EMPIAR-11784

SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state [multiple data sets in EER format]

Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

DOI: 10.1038/s41586-022-05114-0

Related EMDB entry:

EMD-14501

Deposited:

2023-11-10

Released:

2024-02-06

Last modified:

2024-02-06

Imageset size:

7.72 TB

Imageset DOI:

10.6019/EMPIAR-11784

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

8541

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.45, 0.45)

Details:

Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.

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2. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

8199

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.45, 0.45)

Details:

Files:

This entry cannot be downloaded from PDBj.

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3. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7344

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.45, 0.45)

Details:

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7890

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.45, 0.45)

Details:

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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5. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

8541

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.45, 0.45)

Details:

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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