EMPIAR-11781
SpCas9 bound to 14 nucleotide complementary DNA substrate [multiple data sets in TIFF and DM4 formats]
Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

Related EMDB entry:
Deposited:
2023-11-10
Released:
2023-12-11
Last modified:
2023-12-11
Imageset size:
2.07 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of SpCas9 bound to 14 nucleotide complementary DNA substrate
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7881
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.325, 0.325)
Details:
Unaligned multi-frame movies with each containing 36 frames per movie collected at 300 kV in super-resolution counting mode. Total accumulated electron exposure on the specimen level corresponds to 66.8 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU.
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2. Gain reference
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
1
Image size:
(11520, 8184)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.325, 0.325)
Details:
Gain reference
Files:
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Files:
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