EMPIAR-11779
SpCas9 bound to 6 nucleotide complementary DNA substrate [multiple data sets in TIFF and DM4 formats]
Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

Related EMDB entry:
Deposited:
2023-11-10
Released:
2024-01-23
Last modified:
2024-01-23
Imageset size:
1.53 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of SpCas9 bound to 6 nucleotide complementary DNA substrate
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5988
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.325, 0.325)
Details:
Unaligned multi-frame movies with each containing 37 frames per movie collected at 300 kV in super-resolution counting mode. Total accumulated electron exposure on the specimen level corresponds to 64.2 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with EPU.
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2. Gain reference
Category:
micrographs - single frame
Image format:
DM4
No. of images or tilt series:
1
Image size:
(11520, 8184)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.325, 0.325)
Details:
Gain reference
Files:
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Files:
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)