EMPIAR-11774 - Single particle cryo-EM dataset of homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum following reaction with the 2-oxoglutarate analogue succinyl phosphonate [16647 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-11774

Single particle cryo-EM dataset of homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum following reaction with the 2-oxoglutarate analogue succinyl phosphonate [16647 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

High resolution cryo-EM and crystallographic snapshots of the actinobacterial two-in-one 2-oxoglutarate dehydrogenase

Yang L, Wagner T, Mechaly A, Boyko A, Bruch EM, Megrian D, Gubellini F, Alzari PM, Bellinzoni M

Nature communications 14 (2023)

PMID: 37563123

DOI: 10.1038/s41467-023-40253-6

Related EMDB entry:

EMD-17455

Deposited:

2023-07-06

Released:

2023-12-04

Last modified:

2023-12-04

Imageset size:

6.22 TB

Imageset DOI:

10.6019/EMPIAR-11774

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of C. glutamicum 2-oxoglutarate dehydrogenase OdhA following reaction with the oxoglutarate analogue succinyl phosphonate

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

16647

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.86, 0.86)

Details:

Raw dataset of 16647 uncorrected multi-frame micrographs, each composed of 40 fractions accumulating a dose of 40 electrons per squared Angstrom in a 1.4 s exposure. Pixel size 0.86 Angstroms.

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Citations

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