α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected in two different datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included for each dataset in .dm4 format; 20220516_K3GainRefx1m3.dm4 is used to reference movies 20220517**.tif and 20220522_K3GainRefx1m3.dm4 is used to reference movies 20220525**.tif.
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α4β7 integrin bound to MadCAM-1: 3223 unaligned uncorrected movies collected over 2 datasets (803 + 2420) were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.
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Stacks of 188443 particles selected after sequential 2D and 3D classification contributing to final reconstruction of α4β7 integrin bound to MadCAM-1. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.
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