EMPIAR-11764 - Single-particle cryo electron microscopy of apo-Integrin a4b7 in the compact state [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11764

Single-particle cryo electron microscopy of apo-Integrin a4b7 in the compact state [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins

Hollis JA, Malik HS, Campbell MG, Chan MC

(---)

Related EMDB entry:

EMD-42607

Deposited:

2023-11-03

Released:

2025-07-16

Last modified:

2025-07-16

Imageset size:

1.41 TB

Imageset DOI:

10.6019/EMPIAR-11764

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame movies of clasped apo-integrin a4b7 in the compact state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1363

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.561, 0.561)

Details:

α4β7 integrin in the apo state: 1363 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The movies contain 100 frames and the super resolution pixel size is 0.561 Å/px. The total dose per movie is ~50 e/Å^2. The gain reference is also included as a .dm4 file.

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2. Dose-weighted aligned micrographs of clasped apo-integrin a4b7 in the compact state

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1363

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

α4β7 integrin in the apo state: 1363 unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.

Files:

This entry cannot be downloaded from PDBj.

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3. Dose-weighted aligned particle stacks of clasped apo-integrin a4b7 in the compact state

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

239117

Image size:

(328, 328)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

Stacks of 239117 particles selected after sequential 2D and 3D classification contributing to final reconstruction of α4β7 integrin in the apo state. Unaligned uncorrected movies were recorded automatically using SerialEM on a K3 camera in super-resolution mode on a Glacios microscope operated at 200 kV (Cs=2.7). The total dose per micrograph is ~50 e/Å^2. Images were dose-weighted and motion-corrected in MotionCor2 via the RELION GUI. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.122.

Files:

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