EMPIAR-11754 - SPA cryo-EM micrographs of chicken ubiquitinated FANCD2-FANCI3D with ds 44-bpDNA [6515 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-11754

SPA cryo-EM micrographs of chicken ubiquitinated FANCD2-FANCI3D with ds 44-bpDNA [6515 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination

Sijacki T, Alcón P, Chen ZA, McLaughlin SH, Shakeel S, Rappsilber J, Passmore LA

Nature structural & molecular biology 29 (2022) 881-890

PMID: 36050501

DOI: 10.1038/s41594-022-00820-9

Related EMDB entry:

EMD-15103

Deposited:

2023-10-30

Released:

2024-01-23

Last modified:

2024-01-23

Imageset size:

866.43 GB

Imageset DOI:

10.6019/EMPIAR-11754

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry (FoilHole*.mrc) were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   3,623,722,900 kilobytes
Compressed size:   908,522,525 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. multi-frame micrographs of the ubiquitinated ggD2I3D complex with 44-bp dsDNA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

6515

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.145, 1.145)

Details:

---

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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