EMPIAR-11711 - Cryo-EM micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583 [4500 multi-frame micrographs composed of 2051 frames each in EER format]

EMPIAR-11711

Cryo-EM micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583 [4500 multi-frame micrographs composed of 2051 frames each in EER format]

Publication:

Structural basis for a Polθ helicase small-molecule inhibitor revealed by cryo-EM

Ito F, Li Z, Minakhin L, Chandramouly G, Tyagi M, Betsch R, Krais JJ, Taberi B, Vekariya U, Calbert M, Skorski T, Johnson N, Chen XS, Pomerantz RT

Nature communications 15 (2024)

PMID: 39143110

DOI: 10.1038/s41467-024-51351-4

Related EMDB entry:

EMD-44765 EMD-44766

Deposited:

2023-09-18

Released:

2024-08-29

Last modified:

2024-08-29

Imageset size:

5.41 TB

Imageset DOI:

10.6019/EMPIAR-11711

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4500

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.92, 0.92)

Details:

We recommend dividing the total frames into 40 frame movies for motion correction. The gain reference image is in the root folder, it does not need to be flipped or rotated.

Files:

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

See all citations

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