EMPIAR-11711 - Cryo-EM micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583 [4500 multi-frame micrographs composed of 2051 frames each in EER format]

EMPIAR-11711

Cryo-EM micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583 [4500 multi-frame micrographs composed of 2051 frames each in EER format]

Publication:

Structural basis for a Polθ helicase small-molecule inhibitor revealed by cryo-EM

Ito F, Li Z, Minakhin L, Chandramouly G, Tyagi M, Betsch R, Krais JJ, Taberi B, Vekariya U, Calbert M, Skorski T, Johnson N, Chen XS, Pomerantz RT

Nature communications 15 (2024)

PMID: 39143110

DOI: 10.1038/s41467-024-51351-4

Related EMDB entry:

EMD-44765 EMD-44766

Deposited:

2023-09-18

Released:

2024-08-29

Last modified:

2024-08-29

Imageset size:

4.92 TB

Imageset DOI:

10.6019/EMPIAR-11711

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human DNA polymerase θ helicase domain bound to inhibitor AB25583

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4500

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.92, 0.92)

Details:

We recommend dividing the total frames into 40 frame movies for motion correction. The gain reference image is in the root folder, it does not need to be flipped or rotated.

Files:

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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