EMPIAR-11696 - CryoEM micrographs collected on a RAD51-ADP filament sample [9214 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11696

CryoEM micrographs collected on a RAD51-ADP filament sample [9214 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

A metal ion-dependent mechanism of RAD51 nucleoprotein filament disassembly

Appleby R, Bollschweiler D, Chirgadze DY, Joudeh L, Pellegrini L

iScience 26 (2023)

PMID: 37216117

DOI: 10.1016/j.isci.2023.106689

Related EMDB entry:

EMD-16224

Deposited:

2023-09-27

Released:

2023-10-06

Last modified:

2023-10-06

Imageset size:

1.54 TB

Imageset DOI:

10.6019/EMPIAR-11696

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Micrographs of wildtype RAD51 filaments bound with ADP in the presence of dsDNA incl. gain reference

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9214

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Data were collected using a Titan Krios G3 microscope fitted with a K3 detector operating at 300 keV. Micrographs were binned 2x in EPU prior to export giving a pixel size of 0.652A.

Gain reference file for 2x binned data:
data/20220711-130kx-K3-18210048_Gain_Ref.x1.m1-bin2.mrc

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Citations

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